DELFIA® assays bring convenience in monoclonal anitbody development
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چکیده
By using sensitive DELFIA immunoassays for screening one can be confident that all the antibody-producing hybrids can be found. The ease, sensitivity and versatility of this assay makes it possible to test the hybridoma supernatants against different antigens in parallel. The assays can be optimized to fulfil specific needs for convenience; lanthanide-labelled compounds and buffers are commercially available or specific reagents can be prepared to meet users’ individual needs.
منابع مشابه
DELFIA Assays: Flexible and Sensitive Tools for Monoclonal Antibody Development
DELFIA® immunoassays are particularly well suited for discovery of specific, high-affinity monoclonal antibodies (mAbs), especially at the low concentrations that may be present in hybridoma supernatants. An assay used in these conditions must be sensitive, specific, reliable, reproducible and easy enough to handle 1,000 to 1,500 samples in one batch. DELFIA time-resolved fluorescence (TRF) ass...
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